SEED SOWER
Pending Patents Published November 2, 2023
Welcome to the weekly edition of Seed Sower, which will share a list of pending patents published the week before by the U.S. Patent and Trademark Office. These patent applications will cover seed-bearing plants and non-transgenic plant breeding methods only.
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LETTUCE NAMED CARRIZO
CUCUMBER HYBRID SVCS0951 AND PARENTS THEREOF
TOMATO HYBRID SVTM9023
INBRED CORN LINE KW4ER1723
INBRED CORN LINE KW4ER1502
INBRED CORN LINE KW7FW1507
VARIETY CORN LINES
SOYBEAN VARIETIES '2984004', '2987416', '2987413', AND '2987331'
A TOMATO PLANT COMPRISING DOMINANT RESISTANCE GENES TO TOMATO BROWN RUGOSE FRUIT VIRUS
TRANSGENIC SOYBEAN EVENT GM_CSM63714 AND METHODS FOR DETECTION AND USES THEREOF
Leucadendron Plant Named 'AFTERBURNER'
This list includes the titles of all U.S. patent applications published November 2, 2023 with the following Cooperative Plant Classification:
A01H: New plants or {non-transgenic} processes for obtaining them; plant reproduction by tissue culture techniques | 6: Angiosperms, i.e. Flowering plants, characterised by their botanic taxonomy
Patents can have more than one classification. There may be other classifications of interest to plant breeders. To see all classifications for A01H, click here.
To assist the reader in understanding each patent application, each title below is followed by the application's abstract and its independent claims. The abstract is a short summary of the invention that allows the reader to understand the gist of disclosed invention. Independent (standalone) claims define the scope of the patent protection being sought. Independent claims may also be followed by one or more dependent claims, which are not listed here in the interest of brevity. To see the application’s complete list of claims:
- Click the patent title to open the application in Patent Center.
- Click “Download PDF.”
- Find the “claims” section, usually located at the end of the document.
Some applications are excluded from Pre-Grant Publication. Those applications include: Applications recognized by the Office as no longer pending; applications that are national security classified (see 37 CFR 5.2(c)), subject to a secrecy order under 35 U.S.C. 181, or under national security review; applications that have been issued as a patent in sufficient time to be removed from the publication process; and applications filed with a nonpublication request in compliance with 37 CFR 1.213(a). See MPEP § 1122-1124.
Members of the public have the option of filing third party submissions of prior art to pending patent applications. Prior art may be any evidence that what is claimed in the patent application has already been publicly described. To learn more about third party preissuance submissions, click here.
The Seed Liaison welcomes your feedback, questions, and concerns at seedliaison@usda.gov.
LETTUCE NAMED CARRIZO
Applicants: Vilmorin-Mikado USA, Inc.
Abstract: Novel lettuce, such as lettuce designated CARRIZO is disclosed. In some embodiments, the disclosure relates to the seeds of lettuce designated CARRIZO, to the plants and plant parts of lettuce designated CARRIZO, and to methods for producing a lettuce plant by crossing the lettuce designated CARRIZO with itself or another lettuce plant. The disclosure further relates to methods for producing a lettuce plant containing in its genetic material one or more transgenes and to the transgenic plants produced by that method and to methods for producing other lettuce plants derived from the lettuce designated CARRIZO.
Independent Claims:
1. A seed of lettuce designated CARRIZO, wherein a representative sample of seed of said lettuce has been deposited under NCIMB No.______.
4. A lettuce plant, a plant part thereof, or a plant cell thereof, wherein the lettuce plant or a plant regenerated from the plant part or the plant cell has all the physiological and morphological characteristics of lettuce designated CARRIZO deposited under NCIMB No.______.
18. A method of producing a plant of lettuce designated CARRIZO comprising at least one desired trait, the method comprising introducing a single locus conversion conferring the desired trait into lettuce designated CARRIZO, whereby a plant of lettuce designated CARRIZO comprising the desired trait is produced.
24. A method of introducing a desired trait into lettuce CARRIZO, said method comprising the steps of: (a) crossing a lettuce designated CARRIZO plant grown from a seed of lettuce designated CARRIZO deposited under NCIMB No.______, with another lettuce plant that comprises a desired trait to produce F1 progeny plants; (b) selecting one or more progeny plants that have the desired trait to produce selected progeny plants; (c) crossing the selected progeny plants with the lettuce designated CARRIZO plants to produce backcross progeny plants; (d) selecting for the backcross progeny plants that have the desired trait and essentially all the physiological and morphological characteristics of lettuce designated CARRIZO deposited under NCIMB No.______ to produce selected backcross progeny plants; and (e) repeating steps (c) and (d) at least one time in succession to produce selected backcross progeny plants that comprise the desired trait and essentially all of the physiological and morphological characteristics of lettuce designated CARRIZO deposited under NCIMB No.______.
CUCUMBER HYBRID SVCS0951 AND PARENTS THEREOF
Applicants: Seminis Vegetable Seeds, Inc.
Abstract: The invention provides seeds and plants of cucumber hybrid SVCS0951 and cucumber inbred line ASL-M319-0721GY. The invention thus relates to the plants, seeds, plant parts, and tissue cultures of cucumber hybrid SVCS0951 and cucumber inbred line ASL-M319-0721GY and to methods for producing a cucumber plant produced by crossing such plants with themselves or with another plant, such as a cucumber plant of another genotype. The invention further relates to seeds and plants produced by such crossing. The invention further relates to plants, seeds, plant parts, and tissue cultures of cucumber hybrid SVCS0951 and cucumber inbred line ASL-M319-0721GY comprising introduced beneficial or desirable traits.
Independent Claims:
1. A cucumber plant comprising at least a first set of the chromosomes of cucumber line ASL-M319-0721GY, a sample of seed of said line having been deposited under NCMA Accession No. 202203088.
15. A cucumber plant comprising at least a first set of the chromosomes of cucumber line ASL-M319-0721GY, a sample of seed of said line having been deposited under NCMA Accession No. 202203088, further comprising a transgene.
17. A cucumber plant comprising at least a first set of the chromosomes of cucumber line ASL-M319-0721GY, a sample of seed of said line having been deposited under NCMA Accession No. 202203088, further comprising a single locus conversion.
TOMATO HYBRID SVTM9023
Applicants: Seminis Vegetable Seeds, Inc.
Abstract: The invention provides seeds and plants of tomato hybrid SVTM9023. The invention thus relates to the plants, seeds, plant parts, and tissue cultures of tomato hybrid SVTM9023 and to methods for producing a tomato plant produced by crossing such plants with themselves or with another plant, such as a tomato plant of another genotype. The invention further relates to seeds and plants produced by such crossing. The invention further relates to plants, seeds, plant parts, and tissue cultures of tomato hybrid SVTM9023 comprising introduced beneficial or desirable traits.
Independent Claims:
1. A tomato plant of tomato hybrid SVTM9023, wherein said plant comprises a first set of chromosomes and a second set of chromosomes, wherein said first set of chromosomes is a set of the chromosome of tomato line PSQ-9Z15-1572V, and said second set of chromosome is a set of the chromosomes of [[or ]]tomato line PSQ-9Z15-3778V, a sample of seed of said lines having been deposited under NCMA Accession No. 202006016 and NCMA Accession No. 202006017, respectively.
15. A tomato plant of tomato hybrid SVTM9023, wherein said plant comprises a first set of chromosomes and a second set of chromosomes, wherein said first set of chromosomes is a set of the chromosomes of tomato line PSQ-9Z15-1572V and said second set of chromosomes is a set of the chromosomes of tomato line PSQ-9Z15-3778V, a sample of seed of said lines having been deposited under NCMA Accession No. 202006016 and NCMA Accession No. 202006017, respectively, further comprising a transgene.
17. A tomato plant of tomato hybrid SVTM9023, wherein said plant comprises a first set of chromosomes and a second set of chromosomes, wherein said first set of chromosomes is a set of the chromosomes of tomato line PSQ-9Z15-1572V and said second set of chromosomes is a set of the chromosomes of tomato line PSQ-9Z15-3778V, a sample of seed of said lines having been deposited under NCMA Accession No. 202006016 and NCMA Accession No. 202006017, respectively, further comprising a single locus conversion.
INBRED CORN LINE KW4ER1723
Applicants: KWS SAAT SE & Co. KGaA
Abstract: Inbred corn line, designated KW4ER1723, are disclosed. The disclosure relates to the seeds of inbred corn line KW4ER1723, to the plants and plant parts of inbred corn line KW4ER1723 and to methods for producing a corn plant, either inbred or hybrid, by crossing inbred corn line KW4ER1723 with itself or another corn line. The disclosure also relates to products produced from the seeds, plants, or parts thereof, of inbred corn line KW4ER1723 and/or of the hybrids produced using the inbred as a parent. The disclosure further relates to methods for producing a corn plant containing in its genetic material one or more transgenes and to the transgenic plants produced by that method and to methods for producing other corn lines derived from inbred corn line KW4ER1723.
Independent Claims:
1. A seed of inbred corn line designated KW4ER1723, wherein a representative sample of seed of said line was deposited under ATCC Accession No. PTA-______.
3. A corn plant, or a part thereof, having all of the physiological and morphological characteristics of inbred corn line KW4ER1723, wherein a representative sample of seed of said line was deposited under ATCC Accession No. PTA-______.
9. A method for producing inbred corn line KW4ER1723, wherein a representative sample of seed of said line was deposited under ATCC Accession No. PTA-______, the method comprising: (a) planting a collection of seeds comprising seed of a hybrid, one of whose parents is inbred corn line KW4ER1723, the collection also comprising seed of the inbred corn line; (b) growing plants from the collection of seeds; (c) identifying the plants having all of the physiological and morphological characteristics of inbred corn line KW4ER1723 as inbred parent plants; (d) controlling pollination of the inbred parent plants in a manner that preserves the homozygosity of the inbred parent plants; and (e) harvesting the resultant seed and thereby producing an inbred corn line having all of the physiological and morphological characteristics of inbred corn line KW4ER1723.
21. A method of introducing one or more desired traits into inbred corn line KW4ER1723, the method comprising: (a) crossing inbred corn line KW4ER1723 plants grown from inbred corn line KW4ER1723 seeds, wherein a representative sample of seed of the line was deposited under ATCC Accession No. PTA-______, with plants of another corn line that comprise one or more desired traits to produce progeny plants, wherein the one or more desired traits are selected from the group consisting of male sterility, male fertility, herbicide resistance, insect resistance, disease resistance, waxy starch, water stress tolerance, increased amylose starch and increased digestibility; (b) selecting progeny plants that have the one or more desired traits to produce selected progeny plants; (c) crossing the selected progeny plants with the inbred corn line KW4ER1723 plants to produce backcross progeny plants; (d) selecting for backcross progeny plants that have the one or more desired traits to produce selected backcross progeny plants; and (e) repeating steps (c) and (d) one or more times in succession to produce selected second or higher backcross progeny plants that comprise the one or more desired traits and all of the physiological and morphological characteristics of inbred corn line KW4ER1723.
INBRED CORN LINE KW4ER1502
Applicants: KWS SAAT SE & Co. KGaA
Abstract: Inbred corn line, designated KW4ER1502, are disclosed. The disclosure relates to the seeds of inbred corn line KW4ER1502, to the plants and plant parts of inbred corn line KW4ER1502 and to methods for producing a corn plant, either inbred or hybrid, by crossing inbred corn line KW4ER1502 with itself or another corn line. The disclosure also relates to products produced from the seeds, plants, or parts thereof, of inbred corn line KW4ER1502 and/or of the hybrids produced using the inbred as a parent. The disclosure further relates to methods for producing a corn plant containing in its genetic material one or more transgenes and to the transgenic plants produced by that method and to methods for producing other corn lines derived from inbred corn line KW4ER1502.
Independent Claims:
1. A seed of inbred corn line designated KW4ER1502, wherein a representative sample of seed of said line was deposited under ATCC Accession No. PTA-______.
3. A corn plant, or a part thereof, having all of the physiological and morphological characteristics of inbred corn line KW4ER1502, wherein a representative sample of seed of said line was deposited under ATCC Accession No. PTA-______.
9. A method for producing inbred corn line KW4ER1502, wherein a representative sample of seed of said line was deposited under ATCC Accession No. PTA-______, the method comprising: (a) planting a collection of seeds comprising seed of a hybrid, one of whose parents is inbred corn line KW4ER1502, the collection also comprising seed of the inbred corn line; (b) growing plants from the collection of seeds; (c) identifying the plants having all of the physiological and morphological characteristics of inbred corn line KW4ER1502 as inbred parent plants; (d) controlling pollination of the inbred parent plants in a manner that preserves the homozygosity of the inbred parent plants; and (e) harvesting the resultant seed and thereby producing an inbred corn line having all of the physiological and morphological characteristics of inbred corn line KW4ER1502.
21. A method of introducing one or more desired traits into inbred corn line KW4ER1502, the method comprising: (a) crossing inbred corn line KW4ER1502 plants grown from inbred corn line KW4ER1502 seeds, wherein a representative sample of seed of the line was deposited under ATCC Accession No. PTA-______, with plants of another corn line that comprise one or more desired traits to produce progeny plants, wherein the one or more desired traits are selected from the group consisting of male sterility, male fertility, herbicide resistance, insect resistance, disease resistance, waxy starch, water stress tolerance, increased amylose starch and increased digestibility; (b) selecting progeny plants that have the one or more desired traits to produce selected progeny plants; (c) crossing the selected progeny plants with the inbred corn line KW4ER1502 plants to produce backcross progeny plants; (d) selecting for backcross progeny plants that have the one or more desired traits to produce selected backcross progeny plants; and (e) repeating steps (c) and (d) one or more times in succession to produce selected second or higher backcross progeny plants that comprise the one or more desired traits and all of the physiological and morphological characteristics of inbred corn line KW4ER1502.
INBRED CORN LINE KW7FW1507
Applicants: KWS SAAT SE & Co. KGaA
Abstract: Inbred corn line, designated KW7FW1507, are disclosed. The disclosure relates to the seeds of inbred corn line KW7FW1507, to the plants and plant parts of inbred corn line KW7FW1507 and to methods for producing a corn plant, either inbred or hybrid, by crossing inbred corn line KW7FW1507 with itself or another corn line. The disclosure also relates to products produced from the seeds, plants, or parts thereof, of inbred corn line KW7FW1507 and/or of the hybrids produced using the inbred as a parent. The disclosure further relates to methods for producing a corn plant containing in its genetic material one or more transgenes and to the transgenic plants produced by that method and to methods for producing other corn lines derived from inbred corn line KW7FW1507.
Independent Claims:
1. A seed of inbred corn line designated KW7FW1507, wherein a representative sample of seed of said line was deposited under ATCC Accession No. PTA-______.
3. A corn plant, or a part thereof, having all of the physiological and morphological characteristics of inbred corn line KW7FW1507, wherein a representative sample of seed of said line was deposited under ATCC Accession No. PTA-______.
9. A method for producing inbred corn line KW7FW1507, wherein a representative sample of seed of said line was deposited under ATCC Accession No. PTA-______, the method comprising: (a) planting a collection of seeds comprising seed of a hybrid, one of whose parents is inbred corn line KW7FW1507, the collection also comprising seed of the inbred corn line; (b) growing plants from the collection of seeds; (c) identifying the plants having all of the physiological and morphological characteristics of inbred corn line KW7FW1507 as inbred parent plants; (d) controlling pollination of the inbred parent plants in a manner that preserves the homozygosity of the inbred parent plants; and (e) harvesting the resultant seed and thereby producing an inbred corn line having all of the physiological and morphological characteristics of inbred corn line KW7FW1507.
21. A method of introducing one or more desired traits into inbred corn line KW7FW1507, the method comprising: (a) crossing inbred corn line KW7FW1507 plants grown from inbred corn line KW7FW1507 seeds, wherein a representative sample of seed of the line was deposited under ATCC Accession No. PTA-______, with plants of another corn line that comprise one or more desired traits to produce progeny plants, wherein the one or more desired traits are selected from the group consisting of male sterility, male fertility, herbicide resistance, insect resistance, disease resistance, waxy starch, water stress tolerance, increased amylose starch and increased digestibility; (b) selecting progeny plants that have the one or more desired traits to produce selected progeny plants; (c) crossing the selected progeny plants with the inbred corn line KW7FW1507 plants to produce backcross progeny plants; (d) selecting for backcross progeny plants that have the one or more desired traits to produce selected backcross progeny plants; and (e) repeating steps (c) and (d) one or more times in succession to produce selected second or higher backcross progeny plants that comprise the one or more desired traits and all of the physiological and morphological characteristics of inbred corn line KW7FW1507.
VARIETY CORN LINES
Applicants: SYNGENTA CROP PROTECTION AG
Abstract: The present invention provides an inbred corn line designated GIE2002, IID6072, LFF7352, LJC7589, and LJ16305, methods for producing a corn plant by crossing plants of the inbred line GIE2002, IID6072, LFF7352, LJC7589, and LJI6305 with plants of another corn plant. The invention further encompasses all parts of inbred corn line GIE2002, IID6072, LFF7352, LJC7589, and LJ16305, including culturable cells. Additionally provided herein are methods for introducing transgenes into inbred corn line GIE2002, IID6072, LFF7352, LJC7589, and LJ16305, and plants produced according to these methods.
Independent Claims:
1. A seed of maize variety GIE2002, IID6072, LFF7352, LJC7589, and LJ16305, wherein representative seed of said maize variety GIE2002, 1106072, LFF7352, LJC7589, and LJ16305 has been deposited under ATCC Accession Number.
2. A plant of maize variety GIE2002, IID6072, LFF7352, LJC7589, and LJ16305, wherein representative seed of said maize variety GIE2002, 1106072, LFF7352, LJC7589, and LJ16305 has been deposited under ATCC Accession Number.
8. A converted seed, plant, plant part or plant cell of maize variety GIE2002, IID6072, LFF7352, LJC7589, and LJ16305, representative seed of the maize variety GIE2002, 1106072, LFF7352, LJC7589, and LJ16305 having been deposited under ATCC accession number PTA, wherein the converted seed, plant, plant part or plant cell comprises a locus conversion, and wherein the plant or a plant grown from the converted seed, plant part or plant cell comprises the locus conversion and otherwise comprises the phenotypic characteristics of maize variety GIE2002, 1106072, LFF7352, LJC7589, and LJ16305 listed in Table 1 when grown under the same environmental conditions.
15. A process of introducing an additional trait into maize plant GIE2002, 1106072, LFF7352, LJC7589, and LJ16305 comprising: (a) crossing GIE2002, 1106072, LFF7352, LJC7589, and LJ16305 plants grown from GIE2002, IID6072, LFF7352, LJC7589, and LJ16305 seeds, representative seeds deposited under ATCC Accession Number, with plants of another maize variety that comprise an additional trait to produce hybrid progeny plants, (b) selecting hybrid progeny plants that have the additional trait to produce selected hybrid progeny plants; (c) crossing the selected progeny plants with the GIE2002, IID6072, LFF7352, LJC7589, and LJ16305 plants to produce backcross progeny plants; (d) selecting for backcross progeny plants that have the additional trait to produce selected backcross progeny plants; and (e) repeating steps (c) and (d) at least three or more times to produce backcross progeny plants that comprise the additional trait and all of the physiological and morphological characteristics of maize inbred plant GIE2002, 1106072, LFF7352, LJC7589, and LJ16305 when grown in the same environmental conditions.
SOYBEAN VARIETIES '2984004', '2987416', '2987413', AND '2987331'
Applicants: ASOCIADOS DON MARIO S.A.
Abstract: New soybean varieties designated ‘2984004’, ‘2987416’, ‘2987413’, and ‘2987331’ are described. ‘2984004’, ‘2987416’, ‘2987413’, and ‘2987331’ are soybean varieties exhibiting stability and uniformity.
Independent Claims:
1. A soybean seed selected from the group consisting of a soybean seed designated as ‘2984004’, representative sample of seed having been deposited under ATCC Accession Number X2; a soybean seed designated as ‘2987416’, representative sample of seed having been deposited under ATCC Accession Number X3; a soybean seed designated as ‘2987413’, representative sample of seed having been deposited under ATCC Accession Number X4; and a soybean seed designated as ‘2987331’, representative sample of seed having been deposited under ATCC Accession Number X5.
17. A method of making a soybean variety selected from the group consisting of ‘2984004’, ‘2987416’, ‘2987413’, and ‘2987331’, said method comprising: selecting seeds from the cross of one ‘2984004’ plant with another ‘2984004’ plant, a sample of ‘2984004’ soybean seed having been deposited under ATCC Accession Number X2; selecting seeds from the cross of one ‘2987416’ plant with another ‘2987416’ plant, a sample of ‘2987416’ soybean seed having been deposited under ATCC Accession Number X3; selecting seeds from the cross of one ‘2987413’ plant with another ‘2987413’ plant, a sample of ‘2987413’ soybean seed having been deposited under ATCC Accession Number X4; or selecting seeds from the cross of one ‘2987331’ plant with another ‘2987331’ plant, a sample of ‘2987331’ soybean seed having been deposited under ATCC Accession Number X5.
A TOMATO PLANT COMPRISING DOMINANT RESISTANCE GENES TO TOMATO BROWN RUGOSE FRUIT VIRUS
Applicants: PHILOSEED LTD.
Abstract: A tomato plant, harboring a partially dominant resistance gene or genes to tomato brown rugose fruit virus (ToBRFV). Such partially dominant resistance was obtained by crossing and back crossing of nine ToBRFV-resistant wild tomato plant lines, each harboring said partially dominant resistance gene or genes and a ToBRFV-susceptible cultivated Solanum lycopersicum. In addition, genetic markers for partially dominant resistance to tomato brown rugose fruit virus (ToBRFV) where identified and found to be related to chromosome 2, and genetic markers for sensitivity to tomato brown rugose fruit virus (ToBRFV) where identified and found to be related to chromosome 11.
Independent Claims:
32. A tomato plant harboring a partially dominant resistance gene or genes to tomato brown rugose fruit virus (ToBRFV) wherein said plant is the offspring of crossing a ToBRFV-resistant wild tomato plant with a ToBRFV-susceptible cultivated tomato plant or a TOBRFV-resistant wild tomato plant with a ToBRFV-susceptible wild tomato plant; and wherein, said ToBRFV-resistant wild tomato plant harbors said partially dominant resistance gene or genes and said ToBRFV-resistant wild tomato plant is selected from a group consisting of LA0107 ( corneliomulleri), LA0361, LA1918, LA2650 (S. habrochaites), LA1938, LA1969, LA2748, LA2755 and LA2931 (S. chilense); and further wherein said ToBRFV-susceptible cultivated tomato plant is Solanum lycopersicum.
35. Genetic markers for the use in identification or selection of tomato plants harboring partially dominant resistance to tomato brown rugose fruit virus (ToBRFV), wherein said markers are configured to be linked to the gene or genes or QTL conferring partially dominant resistance to ToBRFV in said tomato plants;
37. Genetic markers for the use in identification and/or negative selection of tomato plants harboring sensitivity to tomato brown rugose fruit virus (ToBRFV) wherein said markers are configured to be linked to the gene or genes or QTL conferring sensitivity to ToBRFV in said tomato plants.
42. A tomato plant harboring a partially dominant resistance gene or genes and lacking sensitivity to tomato brown rugose fruit virus (ToBRFV), wherein said plant is the offspring of crossing a ToBRFV-resistant wild tomato plant, which also lack sensitivity to ToBRFV, with a ToBRFV-susceptible cultivated tomato plant, or a TOBRFV-resistant wild tomato plant with a ToBRFV-susceptible wild tomato plant; and wherein said ToBRFV-resistant wild tomato plant harbors said partially dominant resistance gene or genes and lacks said sensitivity genes; and wherein said partially dominant resistance gene or genes is on chromosome 2, said sensitivity gene or genes is on chromosome 11.
43. Genetic markers for the use in identification or selection of tomato plants harboring partially dominant resistance and lacking sensitivity to tomato brown rugose fruit virus (ToBRFV), wherein said markers are configured to be linked to the gene or genes or QTL conferring partially dominant resistance to ToBRFV and sensitivity in said tomato plants.
TRANSGENIC SOYBEAN EVENT GM_CSM63714 AND METHODS FOR DETECTION AND USES THEREOF
Applicants: Monsanto Technology LLC
Abstract: A transgenic soybean event, Gm_CSM63714, is provided. Transgenic plant cells, plant parts, plants, seeds, progeny plants, and agricultural and commodity products containing event Gm_CSM63714 are also provided. Recombinant DNA molecules unique to the event Gm_CSM63714, and methods of using and detecting Gm_CSM63714 are also provided. Soybean plants containing the event Gm_CSM63714 exhibit tolerance to benzoic acid auxins such as dicamba; phenoxy auxins such as 2,4-D; inhibitors of glutamine synthetase such as glufosinate; and β-triketone inhibitors of 4-hydroxyphenylpyruvate dioxygenase (HPPD) such as mesotrione.
Independent Claims:
1. A recombinant DNA molecule comprising a nucleotide sequence selected from the group consisting of SEQ ID NO:10, SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, a polynucleotide having a nucleotide sequence that is at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% identical to the full length of SEQ ID NO:10 or the full length of SEQ ID NO: 9, and a complete complement of any of the foregoing.
6. A DNA molecule comprising a polynucleotide segment of sufficient length to function as a DNA probe: a) that hybridizes specifically under stringent hybridization conditions with soybean event Gm_CSM63714 DNA in a sample, wherein detecting hybridization of the DNA molecule under the stringent hybridization conditions is diagnostic for the presence of soybean event Gm_CSM63714 in the sample; or b) specific for detecting in a sample at least one of: i) a 5′ junction sequence between flanking soybean genomic DNA and the transgenic insert of soybean event Gm_CSM63714; ii) a 3′ junction sequence between the transgenic insert of soybean event Gm_CSM63714 and flanking soybean genomic DNA; iii) SEQ ID NO:9; or iv) a fragment of SEQ ID NO:9 comprising a sufficient length of contiguous nucleotides of SEQ ID NO:9 to identify the sequence as a fragment of the transgenic insert of Gm_CSM63714.
11. A pair of DNA molecules comprising a first DNA molecule and a second DNA molecule, wherein the first and the second DNA molecules comprise a fragment of SEQ ID NO:10 or a complement thereof and function as DNA primers when used together in an amplification reaction with DNA comprising soybean event Gm_CSM63714 to produce an amplicon diagnostic for soybean event Gm_CSM63714 in a sample.
34. A method for controlling or preventing weed growth in an area, the method comprising planting soybean comprising event Gm_CSM63714 in the area and applying an effective amount of at least one herbicide selected from the group consisting of dicamba, glufosinate, 2,4-D, a β-triketone HPPD inhibitor, and any combination thereof, to control weeds in the area without injury to the soybean or with less than about 10% injury to the soybean.
38. A method for controlling volunteer soybean comprising soybean event Gm_CSM63714 in an area, the method comprising applying an herbicidally effective amount of at least one herbicide other than dicamba, glufosinate, 2,4-D, or a β-triketone HPPD inhibitor, wherein the herbicide application prevents growth of soybean comprising soybean event Gm_CSM63714.
41. A method of obtaining a seed of a soybean plant or a soybean plant that is tolerant to benzoic acid auxins, phenoxy auxins, inhibitors of glutamine synthetase, β-triketone HPPD inhibitors, or any combination thereof, the method comprising: a) obtaining a population of progeny seed or plants grown therefrom, at least one of which comprises soybean event Gm_CSM63714; and b) identifying at least a first progeny seed or plant grown therefrom that comprises soybean event Gm_CSM63714.
46. A method of determining the zygosity of a soybean plant, plant part, plant seed, or plant cell comprising soybean event Gm_CSM63714, the method comprising: a) contacting a sample comprising DNA derived from the soybean plant, plant part, plant seed, or plant cell with a primer set capable of producing a first amplicon diagnostic for the presence of soybean event Gm_CSM63714 and a second amplicon diagnostic for the wild-type soybean genomic DNA not comprising soybean event Gm_CSM63714; performing a nucleic acid amplification reaction; and detecting the first amplicon and the second amplicon, wherein the presence of both amplicons indicates that the plant, plant part, seed or cell is heterozygous for soybean event Gm_CSM63714, and the presence of only the first amplicon indicates that the plant, plant part, seed, or cell is homozygous for soybean event Gm_CSM63714; or b) contacting a sample comprising DNA derived from the soybean plant, plant part, plant seed, or plant cell with a probe set comprising at least a first probe that specifically hybridizes to soybean event Gm_CSM63714, and at least a second probe that specifically hybridizes to soybean genomic DNA that was disrupted by insertion of the heterologous DNA of soybean event Gm_CSM63714 but does not hybridize to soybean event Gm_CSM63714; and hybridizing the probe set with the sample under stringent hybridization conditions, wherein detecting hybridization of only the first probe under the hybridization conditions is diagnostic for a soybean plant, plant part, seed or plant cell homozygous for soybean event Gm_CSM63714, and wherein detecting hybridization of both the first probe and the second probe under the hybridization conditions is diagnostic for a soybean plant, plant part, seed, or plant cell heterozygous for soybean event.
50. A DNA construct comprising: a) a first expression cassette, a second expression cassette, a third expression cassette and a fourth expression cassette, wherein: the first expression cassette comprises in operable linkage i) ubiquitin (UB3) promoter, leader, and intron sequences from Arabidopsis thaliana, ii) a chloroplast transit peptide coding sequence of APG6 (Albino and Pale Green 6) from Arabidopsis thaliana, iii) a codon-optimized dicamba monooxygenase coding sequence (DMO) from Stenotrophomonas maltophilia, and iv) a 3′ UTR sequence of the aluminum-induced Sali3-2 protein from Medicago truncatula; the second expression cassette comprises in operable linkage i) a promoter and an intron sequence derived from multiple promoter and intron sequences from Arabidopsis thaliana, ii) a codon-optimized phosphinothricin N-acetyltransferase (PAT) coding sequence from Streptomyces viridochromogene, and iii) a 3′ UTR of a small heat shock protein (Hsp20) from Medicago truncatula; the third expression cassette comprises in operable linkage i) polyubiquitin (UBQ10) promoter, leader, and intron sequences from Arabidopsis thaliana, ii) an alpha-ketoglutarate-dependent non-heme iron dioxygenase variant coding sequence (FT_Tv7) from Sphingobium herbicidovorans, and iii) a 3′ UTR sequence of a putative protein from Medicago truncatula, and the fourth expression cassette comprises in operable linkage i) promoter, leader, and intron sequences derived from multiple promoter, leader and intron sequences from Arabidopsis thaliana, ii) a codon-optimized coding sequence of the triketone dioxygenase (TDO) from Oryza sativa, and iii) a 3′ UTR sequence derived from multiple 3′ UTR sequences from Zea mays; b) a first expression cassette, a second expression cassette, a third expression cassette and a fourth expression cassette, wherein: the first expression cassette comprises a dicamba monooxygenase coding sequence; the second expression cassette comprises a phospinothricin N-acetyltransferase (PAT) coding sequence; the third expression cassette comprises an alpha-ketoglutarate-dependent non-heme iron dioxygenase variant coding sequence (FT_Tv7) capable of degrading 2,4-D; and the fourth expression cassette comprises a triketone dioxygenase (TDO) coding sequence; and wherein the DNA construct further comprises at the 5′ or 3′ end of said construct (i) at least 50 contiguous nucleotides of SEQ ID NO: 11 or SEQ ID NO:98; or (ii) at least 50 contiguous nucleotides of SEQ ID NO: 12 or SEQ ID NO:99; or c) a polynucleotide having a sequence that is at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% identical to the full length of SEQ ID NO: 9; and wherein the DNA construct comprises at the 5′ or 3′ end of said construct (i) at least 50 contiguous nucleotides of SEQ ID NO: 11 or SEQ ID NO:98; or (ii) at least 50 contiguous nucleotides of SEQ ID NO: 12 or SEQ ID NO:99.
76. A method of reducing loci for soybean breeding by inserting transgenes at a single genomic location for tolerance to at least three different classes of herbicides.
86. A soybean plant, plant cell, plant part, or plant seed comprising a recombinant DNA construct integrated in chromosome 13, wherein the recombinant DNA construct confers tolerance to at least one herbicide selected from the group consisting of benzoic acidauxins, phenoxy auxins, glutamine synthetase inhibitors, β-triketone HPPD inhibitors, and combinations of any thereof, and wherein the recombinant DNA construct is integrated in a position of said chromosome flanked by at least 50 contiguous nucleotides of SEQ ID NO:11 or SEQ ID NO:98 and 50 contiguous nucleotides of SEQ ID NO:12 or SEQ ID NO:99.
Leucadendron Plant Named 'AFTERBURNER'
Inventor: Luen Miller
Abstract: A new and distinct Leucadendron species cultivar named ‘AFTERBURNER’ is disclosed, characterized by a distinctively low and compact plant habit. Plants are strongly branched with many sprays of flower heads. Floral bracts are creamy yellow with a rosy apical flush. Resistance to root rot has been observed in the new variety. The new cultivar is a Leucadendron, typically suited for ornamental garden use.
Independent Claims:
1. A new and distinct cultivar of Leucadendron plant named ‘AFTERBURNER’ as herein illustrated and described.
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