Johne’s Disease ELISA Approved for Bovine Milk Testing
Anne Parkinson, BS, Serology Section Head
VMRD, Inc. has announced that their Mycobacterium paratuberculosis/Johne’s Disease ELISA Antibody Test Kit has now been approved by the USDA’s Center for Veterinary Biologics (CVB) for testing bovine milk. Validation studies by VMRD to detect antibodies in bovine milk were confirmed in test animals using culture of paired fecal samples to determine their disease status. The sensitivity was determined to be 84.8% with specificity at 82.1% - which is an improvement of 10% over another previously licensed commercial kit. VMRD’s kit is now licensed by the USDA-CVB for bovine serum AND bovine milk, making it an effective tool for herd management of Mycobacterium paratuberculosis/Johne’s disease in cattle by detecting the presence of the organism before it is spread thru milk to susceptible neonates. The ADDL is now offering this ELISA for the same fee as a serum sample at $5.00 per test. Samples for this assay are tested on Tuesdays and can be submitted to the laboratory on the ADDL General Submission Form.
The Ohio ADDL will be closed on Monday, January 17th, 2022 in observance of Martin Luther King Jr. Day.
If you need to contact us regarding an urgent matter, please use our after hours phone number: (888) 456-3405.
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 Employee Update at ADDL
Dominika Jurkovic, PhD, Bacteriology Laboratory Scientist 3
Ashley Sawyer was promoted to Research 3 in the Virology Lab. Ashley graduated with a Master of Science degree in Genetics, Cell and Molecular Biology from the University of Nebraska-Lincoln in 2016. Her graduate research focused on Kaposi’s Sarcoma-Associated Herpesvirus and Human Immunodeficiency Virus infection of the central nervous system. Ashley has been a laboratory scientist for over 2 years working in Molecular Diagnostics, Virology and Next-Generation Sequencing. Her expertise encompasses environmental microbiology, biochemistry, organic chemistry, analytical chemistry and virology. In her spare time, she likes to spend time with her husband and two daughters, travel, sing, play piano, listen to podcasts and read.
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The Molecular Diagnostic section can now perform Tritrichomonas foetus PCR testing of preputial scrapings collected in saline. Please note: If culture AND PCR is needed for an animal, the sample must still be submitted in InPouch TF media. These pouches can be ordered from the Bacteriology Section at 614-728-6305.
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