Ohio ADDL February Newsletter


Ohio Department of Agriculture   -   FEBRUARY 2018

In this issue

- CWD Detection in Captive Deer

- Campylobacteriosis in Humans Linked to Pet Stores

- Whole Genome Sequencing of Influenza Virus

- New Molecular Tests

- Beef Expo

- Cold Weather Shipping

- Holidays

Contact us

Animal Disease Diagnostic Laboratory

8995 East Main Street

Reynoldsburg, OH 43068

Phone: (614) 728-6220

Fax: (614 ) 728-6310



ADDL Hours

Monday - Friday

8:00 am to 5:00 pm

Excluding the following holidays:

  • New Years Day
  • Martin Luther King Day
  • President's Day
  • Memorial Day
  • Independence Day
  • Labor Day
  • Columbus Day
  • Veteran's Day
  • Thanksgiving
  • Christmas

Looking for test results?


We're on the web!

Your feedback is important to us

To provide comments about the newsletter or to get additional information on any covered topic or service content, please contact us.

Click here to subscribe

ADDL Announces Detection of CWD in Captive Deer Facility

Dr. Jeff Hayes, MS, DVM, ADDL Pathology Section Head  

The Ohio Department of Agriculture (ODA) announced on January 12, 2018 that there was a confirmed positive case of Chronic Wasting Disease (CWD) in a captive deer. The positive tissue was identified by personnel at the ADDL and the infection was confirmed by the National Veterinary Services Laboratories (NVSL) in Ames, Iowa. The state is taking quarantine action to control the further spread of the disease and there is no evidence that CWD has affected the wild deer population in the state. 


The positive sample was taken from a single buck on a hunting preserve in Guernsey County and was tested as part of Ohio’s CWD monitoring program for captive white-tailed deer operations. The animal was transferred from a captive breeding facility in Holmes County just days before it was harvested. Both the hunting preserve and the breeding farm are under quarantine and are subject to intensive monitoring and sampling protocols. The quarantine will remain enforced until the state is satisfied that disease transference can no longer occur between captive operations. 


Testing for the protease resistant protein associated with Chronic Wasting Disease infection is a major surveillance activity at the ADDL, with 8,243 captive deer having been tested since 2014, including 2,023 in 2017 and another 251 through January 25 in 2018. Surveillance of Ohio wild deer for CWD is also conducted each year in corroboration with the Ohio Department of Natural Resources. Samples from more than 5,800 wild deer have been tested since 2014, including samples from more than 1,500 deer collected in 2017 to date. More than 29,500 tissues from Ohio deer,  captive and wild, have been examined for CWD infection since 2014. The ADDL will continue partnering with regulatory officials in this important surveillance program.

Campylobacteriosis in Humans Linked to Pet Stores

ADDL Plays Critical Role in Linking Human and Pet Cases

Dr. Bev Byrum, DVM, PhD, ADDL Laboratory Director 

A multistate outbreak of human Campylobacter infections linked to puppies sold through pet stores appears to be continuing. The CDC reports as of January 18, 2018, 109 people in 17 states have laboratory-confirmed infections or symptoms consistent with a Campylobacter infection linked to this outbreak (CDC Web Site). Of the 109 cases, Ohio has 32 (29% of total) cases linked to the outbreak. 



The Ohio Department of Agriculture Animal Disease Diagnostic Laboratory (ADDL), Ohio Department of Health (ODH), Centers for Disease Control and Prevention (CDC), Food and Drug Administration Center of Veterinary Medicine (FDA CVM), the United States Department of Agriculture, Animal and Plant Health Inspection Service (USDA APHIS) a various local health departments have been involved in the outbreak investigation in Ohio. 

The Ohio ADDL has used state-of-the art techniques such as nucleic acid amplification and whole genomic sequencing (WGS) in this investigation. The Ohio ADDL is supported by the FDA Veterinary Laboratory Investigation and Response Network (Vet-LIRN) program in this effort. As a member of this network, the ADDL isolates Campylobacter from puppy fecal samples, followed by WGS. The data are then uploaded to the national NCBI database which allows it to be compared to other animal and human isolates across the US. 


The Vet-LIRN consists of 41 state and university veterinary diagnostic laboratories that partner with FDA to help investigate potential problems with animal feed, animal drugs and potential zoonotic issues, ultimately safeguarding human and animal health. Vet-LIRN also works with referring veterinarians and pet owners to investigate cases of potential foodborne illness in pets. Each investigation is tailored to the presenting case and in some cases requests diagnostic samples from the ill or deceased pets. 


Vet-LIRN Links that may be helpful to you include: 


How to report a pet food complaint

Vet-LIRN Network Procedures for Veterinarians

Vet-LIRN Network Procedures for Owners 


Please contact the ADDL if you have questions or wish to submit samples as part of this investigation at 614-728-6220 or animal@agri.ohio.gov.

Swine Health
Influenza virus

Whole Genome Sequencing of  Influenza Virus Offered at ADDL

Yan Zhang, DVM, PhD, ADDL Virlogy Section Head

Melanie Prarat, MS, ADDL Virology Section Scientist  



Influenza virus strains of H1N1, H1N2, and H3N2 are co-circulating in swine in Ohio. The ADDL offers whole genome sequencing (WGS) services for influenza. This new service allows the laboratory to quickly identify the strain in a herd. In addition, this new test also provides more information including monitoring for emerging influenza strains due to mutation or recombination. Nasal swab and oral fluid samples can be used for whole genome sequencing.  Please let us know if you are interested in WGS and phylogenetic analysis of influenza that is circulating within your herds.


Human infections with influenza A viruses found in swine are rare events, but can occur. Conversely, human flu viruses can infect pigs and can introduce new flu viruses into the swine population. Routine measures like frequent hand washing, avoiding close contact with animals that look or act ill, and avoiding contact with pigs if you are experiencing flu-like symptoms, can reduce the risk of flu transmission between humans and pigs. More information for pork producers and people who work with or raise pigs is available on the CDC's Swine Influenza/Variant Influenza website.


Melanie Prarat, MS, ADDL Virology Section Scientist 

Several new real-time PCR assays are now available at the ADDL:

Brachyspira pilosicoli:  Acceptable sample types are bacterial isolate, feces, fecal swab, intestines 


Lawsonia intracellularis:  Acceptable sample types are feces, fecal swab, intestines


Porcine circovirus type 3:  Acceptable sample types are oral fluids and tissue

All three tests have a 2-5 day turnaround time.  Contact the Laboratory at 614-728-6220 for more information.

Bovine Health

Beef Expo - March 16-18, 2018

The 31st Ohio Beef Expo is happening March 16-18 in Columbus. You can find the Health Requirements for all breeds hereAny testing for the Expo needs to be completed within 30 days of the show to qualify. The ADDL offers testing services that cover the Expo’s health requirements with results provided within 24 hours of sample receipt. Please contact the ADDL at 614-728-6220 for further testing information.

Shipment During Cold Weather

During the cold winter months, once your samples are picked up by FedEx, UPS, or the USPS, they may be exposed to very low temperatures during transit. Bad weather may also delay delivery of samples, even if you used expedited shipping services. A couple helpful hints to remember when shipping samples to the Ohio ADDL in the winter: 

1.  Try to avoid shipping on Fridays and before holidays.  This helps to prevent samples from prolonged exposure to extremely low temperatures. 

2.  Use foam insulated shippers to protect your samples from very low temperatures. 

Using the recommendations above will help assure your samples arrive at our lab in the best possible condition.

President's Day

Lab Holiday Schedule

The Ohio ADDL will be closed on Monday, February 19th, 2018 in observance of President's Day. 


For additional information and resources, please visit our website.  If you need to contact us regarding an urgent matter, please use our after hours phone number: (888) 456-3405.