Ohio ADDL June Newsletter


Ohio Department of Agriculture   -   JUNE 2017

In this issue

- M. hyopneumoniae ELISA

- H. parasuis PCR now offered

- Respiratory disease complex in Holstein heifer calves

- Upcoming Holidays

Contact us

Animal Disease Diagnostic Laboratory

8995 East Main Street

Reynoldsburg, OH 43068

Phone: (614) 728-6220

Fax: (614 ) 728-6310



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8:00 am to 5:00 pm

Excluding the following holidays:

  • New Years Day
  • Martin Luther King Day
  • President's Day
  • Memorial Day
  • Independence Day
  • Labor Day
  • Columbus Day
  • Veteran's Day
  • Thanksgiving
  • Christmas

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Two-Test ELISA now offered for Mycoplasma hyopneumoniae

Melanie Prarat, MS, ADDL Virology Section

In the past, the ADDL offered the IDEXX-ELISA kit for Mycoplasma hyopneumoniae.  We now have a two-test system utilizing the IDEXX ELISA for screening followed by the Oxoid cELISA for any positive sample.  Please contact the Virology Laboratory at 614-728-6220 or Virology@agri.ohio.gov for more information.

Haemophilus parasuis real-time PCR now offered 

Melanie Prarat, MS, ADDL Virology Section

Haemophilus parasuis (Glasser's Disease) causes an acute infectious disease of pigs, characterized by various combinations of joint problems, pneumonia and multisystemic disease.  Although most pigs harbor the organism in their upper respiratory tract, Glasser's Disease is most often observed in three week to four month-old pigs, usually after stressful events such as weaning, environmental changes, commingling, or as coinfection with other pathogens.  In addition to the culture method, the ADDL now offers a real-time PCR assay for rapid detection of H. parasuis DNA in porcine samples including abscesses, fibrin, joint fluid/swab, nasal swab, oral fluid, pericardium, pleura, serum and tonsil.  Contact the Virology Laboratory at 614-728-6220 or Virology@agri.ohio.gov for more information.


Respiratory disease complex in 2 and 3 month old Holstein heifer calves

Dr. Jeff Hayes, DVM, MS, ADDL Pathology Section Head

Fresh and formalin-fixed lung tissue was submitted from two Holstein heifer calves, 2 and 3 months of age, one showing signs of acute interstitial pneumonia, and one showing signs of chronic pneumonia. Consolidation of lung tissue was reported for both calves, with focal pleural adhesions noted in one calf, and hard nodules within affected lung tissue of the other calf. Heavy growth of both Mannheimia haemolytica and Pasteurella multocida was cultured from lung tissue of each heifer. In addition, Histophilus somni was also isolated from the lung of one calf, accompanied by detection of IBR virus antigen by fluorescent antibody test.  The ADDL has a comprehensive bovine respiratory panel involving the disciplines of histopathology, bacteriology, virology and molecular diagnostics. A serum panel is also available for four bovine respiratory pathogens (BVD, IBR, BRSV and PI3). Respiratory panels are also prepared for horses, pigs, sheep and goats. See the ADDL website or contact the ADDL at 614-728-6220 for details (sample types, tests, costs).


Lab Holiday Schedule

The Ohio ADDL will be closed on Tuesday, July 4th, 2017 in observance of Independence Day. 


For additional information and resources, please visit our website.  If you need to contact us regarding an urgent matter, please use our after hours phone number: (888) 456-3405.