Ohio ADDL May 2017 Newsletter


Ohio Department of Agriculture   -   MAY 2017

In this issue

- New Test: Porcine Rotavirus A, B, & C RT-qPCR

- Update: Swine Influenza in Ohio

- Pathology Case Report: Canine Herpesvirus

- eSTEM Interns

- Lab Holiday Schedule

Contact us

Animal Disease Diagnostic Laboratory

8995 East Main Street

Reynoldsburg, OH 43068

Phone: (614) 728-6220

Fax: (614 ) 728-6310



ADDL Hours

Monday - Friday

8:00 am to 5:00 pm

Excluding the following holidays:

  • New Years Day
  • Martin Luther King Day
  • President's Day
  • Memorial Day
  • Independence Day
  • Labor Day
  • Columbus Day
  • Veteran's Day
  • Thanksgiving
  • Christmas

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Rapid Detection of Porcine Rotavirus A, B, and C Now Available

Melanie Prarat, ADDL Virology Section Laboratory Scientist 

Rotaviruses are important causes of diarrhea in animals, including pigs.  Four rotavirus serogroups (A, B, C, E) affect swine.  Serogroup A is the most prevalent but serogroups B and C have been associated with outbreaks.  Serogroup E is not common.  Rotaviruses usually cause diarrhea in pigs between 10 and 40 days of age.  The ADDL now offers a real-time RT-PCR assay for rapid detection of rotavirus serogroups A, B, and C in porcine fecal samples.  Contact the Virology Laboratory at 614-728-6220 for more information.

Update - Swine Influenza in Ohio

Yan Zhang, DVM, PhD, ADDL Virology & Molecular Diagnostics Section Head  

Swine influenza (swine flu) is a respiratory disease of pigs caused by type A influenza viruses.  Swine flu viruses do not usually infect humans, but occasional human infections have occurred.  Swine flu viruses are highly contagious among naïve pigs in pig herds, with high morbidity and usually low mortality.  Swine influenza viruses circulate among swine year round, but most outbreaks occur during the late fall and winter months, similar to outbreaks in humans.  Several type A swine influenza subtypes have been detected during 2016 and 2017 in the Ohio pig populationAmong them, H3N2 is most common, followed by H1N1. H1N2 has been detected but is less common.

To find out more information about swine influenza viruses, visit the CDC's Swine Influenza website.  There is valuable information pertaining to pork producers and people who work with or raise pigs.


Puppy with Canine Herpesvirus Type 1 Infection

Jeff Hayes, DVM, ADDL Pathology Section Head  

An Australian shepherd puppy was recently submitted to the ADDL, from a litter of 4 which had died within hours of acting weak and fading.  Histopathological exam showed focal areas of coagulation necrosis and intranuclear inclusions consistent with canine herpesvirus type 1 (CHV-1) in sections of heart, lung and kidney.  Cardiac myofiber necrosis was evidenced by swelling and hypereosinophilia of sarcoplasm, loss of cross striations, fragmentation, with pyknosis and karyorrhexis of nuclei. Some foci contained mild macrophagic infiltrates as well as nuclear debris. Petechial hemorrhages reported grossly in the kidney, and this was corroborated by many foci of acute tubular epithelial necrosis in each of two sections, many surrounded by a rim of acute hemorrhage. The ADDL has an effective fluorescent antibody assay to detect antigen of CHV-1 in fresh tissues that is inexpensive and rapid.  Submission of fresh tissue in addition to formalin fixed can help provide a rapid diagnosis.  Please contact the Pathology Section (614-728-6220) if you have any questions.


eSTEM High School Students Complete Their Internships at ADDL

Melanie Prarat, ADDL Virology Section Laboratory Scientist 

Three students from Reynoldsburg eSTEM Academy have been interns at the ADDL during their senior high school year.  Kenene Enkossa and Manisha Khatiwada spent time in the Virology and Molecular Diagnostics section learning the science behind virus detection, while Ranjana Thapa interned in the Serology section to learn about diagnostic and export testing. 


The girls are attending the following universities this fall:

Kenene - Miami University (Environmental Science)

Ranjana - The Ohio State University - Newark (Business)

Manisha - The Ohio State University (Pre-Medicine)


We enjoyed teaching these bright students about veterinary diagnostics and we wish them the best of luck in their collegiate endeavors!

Left to Right: Kenene Enkossa, Ranjana Thapa, Manisha Khatiwada

Lab Holiday Schedule

The Ohio ADDL will be closed on Monday, May 29th, 2017 in observance of Memorial Day. 


For additional information and resources, please visit our website.  If you need to contact us regarding an urgent matter, please use our after hours phone number: (888) 456-3405.