Ohio ADDL October Newsletter


Ohio Department of Agriculture   -   OCTOBER 2016

In this issue

- EIA results reporting

- Preparing for PED

- Rabies testing

- New employees

- AAVLD Meeting

- Upcoming training

Contact us

Animal Disease Diagnostic Laboratory

8995 East Main Street

Reynoldsburg, OH 43068

Phone: (614) 728-6220

Fax: (614 ) 728-6310



ADDL Hours

Monday - Friday

8:00 am to 5:00 pm

Excluding the following holidays:

  • New Years Day
  • Martin Luther King Day
  • President's Day
  • Memorial Day
  • Independence Day
  • Labor Day
  • Columbus Day
  • Veteran's Day
  • Thanksgiving
  • Christmas

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Reminder Regarding EIA Test Results Reporting

Starting November 1, the ADDL will provide Official EIA Test Results electronically rather than sending hard copies via the US Postal Service. Results will be available the day the test is reported by e-mail, fax and/or web portal. Please contact the ADDL at (614) 728-6220 if you have any questions.

UPS Holiday Schedule

Samples sent to the lab via UPS delivery may be delayed due to the company's temporary holiday schedule.  Normal delivery times are being delayed 2-3 hours, which impacts the lab's ability to start and complete same-day testing. We will make every effort to complete same-day testing on samples received after noon, however we strongly recommend samples submitted for same-day results be submitted using UPS Next Day Air which guarantees delivery by 10:30 AM. The UPS Holiday schedule will be in effect from now through January.


Preparing for PED This Winter

Melanie Prarat, ADDL Virology Section

As the winter months approach, a decrease in temperature typically coincides with an increase in several swine diseases.  Although outbreaks of swine enteric coronavirus disease (SECD) (including porcine epidemic diarrhea (PED) and swine deltacoronavirus) have been relatively infrequent compared to the 2013-2014 epidemic that killed millions of piglets, the virus remains a concern.  According to the USDA APHIS SECD Situation Report, PED incidence usually starts to increase in mid-November, with peak incidence in February.    


Porcine reproductive and respiratory syndrome (PRRS) and swine influenza outbreaks also become more frequent in the winter months. The ADDL is prepared to assist producers during these times by offering same-day test results for PED, swine deltacoronavirus, PRRS and SIV.      


ADDL Collaborates with Ohio Department of Health for Rabies Testing of Large Animals

Jeff Hayes, DVM, MS, ADDL Pathology Section Head

Removing brain tissue from the heads of large animals can be a challenge. Although rabies testing is done by the Ohio Department of Health (ODH) which is co-located on the ODA campus, the ADDL assists them in brain removal for large animal cases. The ADDL recommends  submitting the entire head to the Ohio ADDL. The Necropsy staff can transfer the brain sample safely to ODH. While there is no cost for the direct fluorescent antibody test to detect rabies virus antigen in brain tissue, the ADDL does charge a fee of $43 for brain removal.


Note the following advantages of utilizing the ADDL.  First, ADDL can retain other portions of the brain for additional testing, following a negative rabies test result from ODH, in order to determine a definitive diagnosis. This may include bacterial culture, fluorescent antibody testing, or PCR assays to detect pathogen nucleic acid. Second, in some cases, other samples than brain are needed to diagnose causes of neurologic clinical signs. ADDL pathologists may need to collect retropharyngeal lymph node for diagnosis of malignant catarrhal fever, or equine herpesvirus type 1. Third, they can also evaluate the brain for gross and histopathologic lesions of polioencephalomalacia, and perform histopathology to detect microscopic lesions of infectious diseases, or in some cases, to document neoplasia in the brain.

ODH Rabies Submission Form

ODA Sample Submission Form

New Employees

Meet Our New Employees

(L to R: Lauren Spadafora, Rocio Nava, Chris McKee


Dr. Lauren Spadafora graduated from the University of Washington in 2015 with a Ph.D in Pathobiology. Prior to joining ADDL's Bacteriology Section as a Laboratory Scientist, she worked with the Ohio Coverdell Stroke Program at the Ohio Department of Health.


Dr. Chris McKee received his Ph.D. in 2010 from the Molecular, Cellular, & Developmental Biology program at The Ohio State University, where he studied the molecular biology of retroviruses.  Chris previously worked at ADDL from 2003-2005 as a Microbiologist and returned this spring as a Laboratory Scientist.


Dr. Rocio Nava graduated from the University of Zulia (Venezuela)  with her DMV in 1991 and with a Master's in Epidemiology in 2005.  She recently joined ADDL as a Laboratory Scientist in the Bacteriology Section.


Welcome to the ADDL team!


ADDL Staff Make Presentations at National AAVLD Meeting

Drs. Bev Byrum, Jeff Hayes, Jing Cui, and Yan Zhang attended the 59th annual meeting of the American Association of Veterinary Laboratory Diagnosticians in Greensboro, North Carolina from October 13-16.  The following presentations were made by ADDL during the meeting:

- A case of tularemia in a gray fox which emphasized the culture and biosafety procedures needed to establish the diagnosis, and the histopathologic aspects of the disease. (Dr. J. Hayes)

- Diagnosis of Brucella canis infection using serologic assay. (Dr. Y. Zhang)

- Outbreak of a pandemic H1N1 influenza virus in a swine herd in Ohio. (Dr. Y. Zhang)

-Comparison of AAVLD and ISO 17025 accreditation standards. (Dr. B. Byrum)

CWD Training Event at Division of Animal Health on November 2, 2016

The ADDL will conduct training for veterinarians wanting to be approved to collect samples for CWD as part of the Cervid Captive Herd Program, on Wednesday, November 2. The training will take place at the Animal Disease Diagnostic Lab, Division of Animal Health (Building 6) on the Ohio Department of Agriculture campus at 8995 East Main Street, Reynoldsburg, Ohio 43068. Training will be from 9 AM until 3 PM, and will include lectures and a wet lab. The training will allow practitioners to develop proficiency in sample collection for Chronic Wasting Disease (CWD) testing. There is no cost for the training.  Please contact Christi Clevenger or Cindy Bodie at (614) 728-6220 by Monday, October 31 to sign up for the training or for more information.


The ADDL will be CLOSED on November 11 (Veteran's Day) and November 24 (Thanksgiving).  If you need to contact us regarding an urgent matter, please use our after hours phone number: (888) 456-3405.