Ohio ADDL August Update


Ohio Department of Agriculture   -   AUGUST 2016

In this issue

- Lab closed Labor Day

- Bovine TB in a beef herd

- BVD/IBR semen testing

- EEE/WNV vaccination

Contact us

Animal Disease Diagnostic Laboratory

8995 East Main Street

Reynoldsburg, OH 43068

Phone: (614) 728-6220

Fax: (614 ) 728-6310



ADDL Hours

Monday - Friday

8:00 am to 5:00 pm

Excluding the following holidays:

  • New Years Day
  • Martin Luther King Day
  • President's Day
  • Memorial Day
  • Independence Day
  • Labor Day
  • Columbus Day
  • Veteran's Day
  • Thanksgiving
  • Christmas

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Labor Day

Lab closed on Monday, September 5th

The Ohio ADDL will be closed on Monday, September 5th, 2016 in observance of Labor Day.

For additional information and resources, please visit our website.  If you need to contact us regarding an urgent matter, please use our after hours phone number: (888) 456-3405.

Thank you and have a great holiday weekend!

Ohio Animal Disease Diagnostic Laboratory

ADDL Assists Indiana and USDA in Confirming Bovine TB in Beef Herd

-Jeff Hayes, DVM, MS, ADDL Pathology Section Head

The ADDL Pathology Section provided surge capacity necropsy service for Indiana and the USDA in the investigation of bovine tuberculosis in a beef herd in southeastern Indiana this past spring. ADDL staff performed necropsy examinations of 11 cattle and submitted samples to NVSL for this investigation.  Culture and PCR analysis at NVSL confirmed the presence of Mycobacterium bovis in ten of the 11 cattle examined at ADDL. Gross lesions consistent with bovine tuberculosis were observed in all ten of the culture/PCR-positive animals. 


In mid-August, Indiana subsequently announced the diagnosis of bovine tuberculosis in a wild white-tailed deer in the same county as the beef herd. This has prompted the Ohio's State Veterinarian, Dr. Tony Forshey, to issue warnings for cattle owners and hunters in Ohio. In particular, cattle owners in southwestern Ohio should closely monitor their herds for symptoms of this bacterial disease, such as lethargy and coughing. Cattle owners should take steps to prevent cattle from having contact with wild animals. Hunters are advised to protect themselves by wearing gloves when field dressing animals and by fully cooking any meat.



Changes to OIE recommendations for IBR and BVD semen testing

Chris McKee, PhD, ADDL Virology Section

Revised guidelines for semen submission           

To ensure consistency with the OIE recommendations, the Virology Section at ADDL is updating procedures for virus isolation and PCR testing of bovine semen.  The updated procedures have increased minimum sample volume requirements for extended semen, as described below:

  • IBR virus isolation:  0.5 mL total extended semen.
  • BVD virus isolation:  1.0 mL total extended semen.
  • BVD PCR:  Three 0.25 mL straws of extended semen.

As always, clients are encouraged to contact the laboratory with any questions they may have regarding sample requirements.

WNV and EEE Vaccination

Jeff Hayes, DVM, MS, ADDL Pathology Section Head

Last Call: Equine clients reminded to vaccinate their horses against west Nile virus (WNV) and eastern equine encephalomyelitis (EEE)

Reports of West Nile virus (WNV) and eastern equine encephalomyelitis (EEE) infections in horses continue to  increase from around the country.  As summer draws to a close, there is still time to remind your clients that their horses can effectively be vaccinated against these viruses that are transmitted primarily by mosquitoes.  Ohio cases of WNV and EEE in horses the past few years have occurred from late August until mid-October. 


The ADDL offers PCR tests for WNV and EEE viruses and can coordinate serology testing with NVSL for blood samples. Please call the ADDL for more information at 614-728-6220, or visit our website test and fees search (pages 12 and 28) at http://www.ohioagriculture.gov/divs/ai/addl/addltests.aspx .