Ohio ADDL March Update


Ohio Department of Agriculture   -   MARCH 2016

In this issue

  • Fee Adjusment Begins May 1st
  • New Biopsy Report Formats
  • Toxicology: Buckeye Intoxication
  • Meet the Staff: Serology Section
  • Virology: Funding Support for SECD Testing Ending

Contact us

Animal Disease Diagnostic Laboratory

8995 East Main Street

Reynoldsburg, OH 43068

Phone: (614) 728-6220

Fax: (614 ) 728-6310



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8:00 am to 5:00 pm

Excluding the following holidays:

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Fee Adjustment Begins May 1st

The Ohio ADDL is constantly looking at opportunities to operate more efficiently so that you, our customer, receive the best service at the best price. We are committed to providing a wide variety of state-of-the-art, quality diagnostic services in an affordable, accurate and timely manner.


At the ADDL, we believe it is important to continue providing the highest quality service we can, and adjusting our fees will allow us to continue to do that. Beginning May 1, 2016, an updated fee list, which will include new tests and services, will be available on our web site. Some of the changes will include:


- Biopsy reports are now offered in a standard (short) and detailed (long) format (See the folllowing article for more information)

- Whole Genome Sequencing is a new service

- Brucella canis serology confirmation and culture are now available

- Many new PCR tests have been added


Visit www.agri.ohio.gov/divs/ai/addl/addltests.aspx after May 1 to review and download the adjusted fee schedule. 


The ADDL takes pride in providing quality, timely and accurate test results for every case we handle. The ADDL employs some of the most talented diagnosticians in the country who are available to assist with your routine and complex diagnostic cases.


We look forward to working with you to promote animal health and protect agricultural, companion animal and public health interests in Ohio and beyond by providing excellence in veterinary diagnostic services.

New Biopsy Report Formats

Beginning May 1, 2016, ADDL clients will have two choices regarding the type of biopsy report they receive, the standard report (diagnosis and comments) or a detailed report (diagnosis and comments plus a detailed histologic description).  The standard report will be provided for biopsy cases, unless a detailed report is requested on the submission form. The fee for the standard report will be $40. The fee for the detailed report will be $55.


Both reports include up to two H&E slides and up to two histochemical stains. These prices pertain to tissues submitted for one animal. Additional H&E or histochemical stain slides are $12, if needed.


An additional new option that is now available for biopsy cases is a Stat Report. These reports will be provided within 48-72 hours for an additional $25.00 fee.  Please denote Stat Report on the submission form if this service is desired.  

Toxicology: Buckeye Intoxication

Buckeye sapling

Reminder: Spring/Fall are the most likely times of inappropriate grazing of poisonous plants


In the spring, plants sprouting from seed or saplings from the previous year often become attractive to livestock for ingestion before the pasture grass becomes lush enough to graze consistently. As a result, cattle and horses will occasionally eat the green plants and become ill. An example is the buckeye tree (Aesculus sp.) where it appears that young growth, sprouts and mature nuts are dangerous. Last fall, a rumen sample from a dead Hereford cow was presented to ODA-ADDL with a concern for buckeye toxicosis being the cause of death. The cow was found dead in a pasture – 1 of 3 affected cows in a group of 12. Another cow showed clinical signs of weakness and muscle tremors, with TPR values of T-101.2°F, P-50, R-50, respectively.  Using a technique of plant microscopic analysis, evidence of the buckeye plant was reported to be present in the rumen contents which confirmed buckeye exposure. For this analysis, submit approximately 1 pound of fresh stomach/rumen contents to ODA-ADDL as soon as possible in a cooler with cold packs (do not freeze). Please select samples for this submission from at least 4 different places in the rumen/stomach contents to increase the likelihood of finding plant fragments.


Signs of buckeye intoxication include incoordination, depression, bizarre behavior, staggering, hypermetria, falling and gastrointestinal upset. Treatment is difficult in cattle and it is best to prevent ingestion. Affected cattle have been known to fall off cliffs or to drown in creeks and ponds; protecting such animals from self-induced injury can be an important aspect of supportive care. In cases where multiple cattle have shown clinical signs of intoxication, they should be moved to a different pasture or barn until the offending plant material can be removed from the area.

ADDL Serology Section

Meet the Staff: Serology Section

Welcome to the Serology Section at the Ohio ADDL!  We are here to provide serology testing requirements covering the intrastate, interstate and international movement of livestock to and from the state of Ohio.  Our section provides the very best testing methods available for regulatory testing, herd monitoring and surveillance, and disease diagnosis.  The section performed in excess of 150,000 tests last year, and is looking forward to increasing that number as the livestock sector in the state grows new opportunities for testing.  We also work closely with our partners at USDA to ensure a smooth transition of export results for our clients moving livestock out of the country on tight international schedules.


The Serology Section is led by Anne Parkinson, and consists of four full-time, highly motivated staff members ready to serve our clientele – Deb Hobbs, Lab Scientist 3; Katie Esbenshade, Lab Scientist 2; Christy Ammons, Lab Scientist 2; and Andrew Cox, Lab Scientist 1.  Our laboratory scientists are all proficiency tested regularly on protocols originating from the National Veterinary Services Laboratory to assure our clients of a high quality test result.  The section is particularly proud of its speedy turn-around times, and strives to provide a positive experience for our clients here in the state of Ohio.  Please don’t hesitate to call and discuss your testing needs – or, for assistance with test result interpretations or complex import/export requirements.  Don’t forget to refer to our website for current test offerings, fees, and new fillable submission forms.

Virology: Funding Support for SECD Testing Ending



In response to the swine enteric coronavirus diseases (SECD) (including porcine epidemic diarrhea virus and swine deltacoronavirus) outbreak of 2014, USDA APHIS provided $26.2 million in emergency funding for monitoring and managing the epidemic.  This funding is anticipated to be exhausted in approximately two months; APHIS will pay for testing of SECD samples received on or before April 30, 2016 and billed to APHIS' Veterinary Services program prior to June 30, 2016.  After this date, swine operations may continue to submit samples to ADDL for diagnostic testing, but reimbursement for testing will no longer be provided.