Ohio ADDL January Update


Ohio Department of Agriculture   -  JANUARY 2016

In this issue

  • AAVLD Accreditation
  • Brucella canis Case Report
  • Bacteriology Update
  • Molecular Diagnostics
  • Pathology Update
  • 28th Annual Ohio Beef Expo

Contact us

Animal Disease Diagnostic Laboratory

8995 East Main Street

Reynoldsburg, OH 43068

Phone: (614) 728-6220

Fax: (614 ) 728-6310



ADDL Hours

Monday - Friday

8:00 am to 5:00 pm

Excluding the following holidays:

  • New Years Day
  • Martin Luther King Day
  • President's Day
  • Memorial Day
  • Independence Day
  • Labor Day
  • Columbus Day
  • Veteran's Day
  • Thanksgiving
  • Christmas

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ADDL maintains AAVLD Accreditation, Recognized as NAHLN Tier 1 Laboratory

Accreditation provides formal recognition of the competency of a laboratory to perform high quality diagnostic testing.The ADDL recently underwent an on-site audit by an AAVLD Accreditation Site Team. The audit included a detailed review of our quality assurance system, standard operating procedures, training records and financial status. Based on the results of the audit, the Ohio ADDL received renewal of our AAVLD Accreditation as a Full Service/All species laboratory for another 5 years. 


Recently, the National Animal Health Laboratory Network (NAHLN) restructured its' network to meet the overall national need. Criteria considered included  (1) lab accreditation, (2) testing capabilities, (3) personnel expertise, (4) facilities and infrastructure status, (5) population density of food animals within a state, (6) geographic location and (7) support provided to the NAHLN program.  As a result, the Ohio ADDL is now recognized as a Tier 1 laboratory, one of only 11 NAHLN labs with this status.  As a Tier 1 NAHLN lab, we will continue to provide surveillance and response services and to expand detection of emerging and zoonotic diseases to protect animal and public health as well as the nation's food supply.


Case Report: Brucella canis in dogs

Four adult female dogs tested  positive for antibodies against Brucella canis at the ADDL using the indirect fluorescent antibody (IFA) and tube agglutination test (TAT) serology tests. The 4 dogs were euthanized and the bodies were submitted for B. canis culture.  Both uterine horns of one dog were diffusely enlarged.  They contained multiple partially decomposed puppies surrounded by large amounts of thick, green, opaque, non-fetid, mucoid material.  Marked necrotizing and mucoid metritis with multiple retained macerated fetuses was diagnosed.  Internal iliac lymph nodes were enlarged, firm, wet, and had multiple prominent follicles (lymphoid hyperplasia).  B. canis was isolated from multiple lymph nodes and spleen of all 4 dogs as well as from the uterus of 3 and ovary of 1 dog.


A second case with six dogs that tested positive for B. canis was also presented to ADDL.  We successfully isolated B. canis from all six animals.  The results from these two cases confirm the test system (IFA and TAT) used at ADDL is highly specific. An animal is considered positive for B. canis infection if both tests are positive. So far, ADDL has tested over 6000 serum samples since the implementation of the B. canis Rule.


We will be present posters and presentations regarding the status of B. canis in Ohio at the upcoming Midwest Veterinary Conference (February 25-28 at the Greater Columbus Convention Center).

Bacteriology: New assays available

New real-time PCR assays are available for detection of Mycoplasma bovis, Mycoplasma bovigenitalium and Ureaplasma diversum.  These assays have been validated at ADDL to be highly sensitive and specific for detection of these pathogens.

Molecular Diagnostics

ADDL now offers PRRS ORF5 sequencing and analysis of porcine serum, tissue, and oral fluid specimens. This test is performed daily with a 3-5 day turnaround time.  Analyses include RFLP pattern interpretation and identification of the isolate as a wild-type strain or one of the commercial vaccines currently used in the United States.

Pathology: Bronchopneumonia in 8 week old barrows with multiple pathogens

Many sick pigs from a sale barn had signs of pneumonia with increased respiratory effort and showed weight loss and lethargy. Fresh and formalin-fixed tissues were submitted for a porcine respiratory diagnostic panel. Differential diagnosis clinically includes Pasteurella, Mycoplasma, Trueperella and viruses. Microscopically, lung tissue from two pigs had marked diffuse acute fibrinopurulent bronchopneumonia with pulmonary abscesses, type II pneumocyte hyperplasia and intralesional bacteria.  These alterations were consistent with PCR detection of North American strain of PRRS virus (ct value of 19) and isolation of multiple bacterial respiratory pathogens of swine:  Pasteurella multocida, Trueperella pyogenes and Actinobacillus suis. Microscopic lesions were not indicative of either swine influenza, porcine circovirus type 2 virus or mycoplasma infections.

28th Annual Ohio Beef Expo

The 28th Annual Ohio Beef Expo is right around the corner, March 18-20, 2016!  For information on health testing requirements for animals going to the Expo, go to:


http://obe2014.correspondnow.com/seedstock-sales-shows  and click on Health Requirements in the upper left hand side.


For directions on sample collection for Tritrichomonas testing, go to:

http://biomeddiagnostics.com/resources/files/100-005%20InPouch-TF%20Insert%20Rev%20H.pdf or call the Bacteriology Laboratory at the Ohio Animal Disease Diagnostic Laboratory at 614-728-6220.